Sentence examples for buffer was obtained by from inspiring English sources

Phosphate buffer was obtained by mixing KH2PO4 and K2HPO4 solutions to receive pH 6.0 or 7.0.

An oxygen depleted solution of 20 m m GSSG in 100 m m sodium phosphate, pH 7.4 [100 m m sodium phosphate buffer was obtained by mixing 3.1 g of NaH2PO4 and 10.9 g of Na2HPO4 (anhydrous) in distilled water to give a final volume of 1 l] was mixed with an excess of NaHS.

Metal-free buffers were obtained by treatment with Chelex-100 resin (BioRad, Hercules, CA) and subsequent filtration through a 0.22-μm filter.

RIPA buffer was obtained from Santa Cruz (Heidelberg, Germany).

Sterile phosphate buffered saline (PBS) buffer was obtained from Life Technologies.

RIPA buffer was obtained from Millipore.

To obtain C-IgG-HIV, 0.20 μg/mL neutralizing HIV-specific IgG (SMI, Stockholm, Sweden) and 20 μg/mL gamma globulin (Pharmacia, Stockholm, Sweden) were added besides the HS containing Veronal buffer, whereas IgG-HIV was obtained by adding only the antibodies.

To obtain C-IgG-HIV, 0.2 μg/mL neutralizing HIV-specific IgG (SMI, Stockholm, Sweden) and 20 μg/mL gamma globulin (Pharmacia, Stockholm, Sweden) were added besides the HS-containing Veronal buffer, whereas IgG-HIV was obtained by adding only the mixture of antibodies.

γ-PGA release into the TRIS buffer solution at each time point was obtained by performing a Pierce Micro bicinchoninic acid (BCA) protein assay.

The heat of dilution was measured by titrating IC into blank buffer, and the net binding heat was obtained by subtracting the dilution heat from the apparent reaction heat.

Fully purified polymer fraction was obtained by elution with buffer containing 80 mM of imidazole with high recovery rate.