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The lysis buffer was not supplemented with proteinase K, containing 50 mM Tris HCl, pH 7.4, 10 mM EDTA, 150 mM NaCl, 1% SDS.
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The mixtures are not supplemented.
The regimens were not supplemented.
(sml-buffer): Query if the current buffer is not a *sml*.
This buffer was also supplemented with (in mmol/L) 10 deoxyglucose, 0.75 sodium dithionate, and 20 lactate.
Wild-type and mutant FICD/HYPE were expressed and purified as EfFIC, except that the lysis buffer was supplemented with 1 mM dithiothreitol (DTT) and 0.02 % Triton X-100 and the other buffers were supplemented with 1 mM DTT.
When optical tweezers were used, the ATP buffer was supplemented with NeutrAvidin-functionalized silica microspheres.
Additional TE buffer was supplemented to make four chains at the same concentration.
Lysis buffer was supplemented with Complete EDTA-free protease inhibitor cocktail (Roche).
The lysis buffer was supplemented with the Complete Protease Inhibitor Cocktail, EDTA-free (Roche).
For immunoprecipitation assays, this lysis buffer was supplemented with 10% glycerol.
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