Sentence examples for buffer was neutralized by from inspiring English sources

After collecting the eluate, the elution buffer was neutralized by adding 200 µl 1 M Tris-HCl pH 8.6.

Fractions eluted with the glycine HCl buffer were neutralized immediately by adding 0.1 volume of 1 M Tris HCl, pH 8.0.

To convert the drug into the hydrophobic form, doxorubicin hydrochloride was neutralized by phosphate buffer solution (0.1 M, pH 8.5) followed by centrifugation.

The supernatant containing ATP was neutralized by Tris/HCl buffer (50 μl, 0.1M, pH 9.0) before it was mixed with the bioluminescent solution and read by a luminometer (Berthold Tube Master Berthold Technologiess).

Briefly, 6 × 10 cells were lysed in 50  μl of alkaline lysis solution (200 mMNaOH, 2 mM EDTA), and the lysate was neutralized by 8  μl of neutralization buffer (1 M HCl, 600 mM Tris pH 8.0).

After DNA denaturation, the remaining HCl was neutralized by incubating cells with 0.1 M borate buffer.

The mixture was vortexed and centrifuged briefly before incubating at room temperature for 3 min. Denatured DNA was neutralized by adding 10 µl of either unmodified or modified buffer N1.

The sample pH was neutralized by addition of the cleavable ICAT reagent kit affinity loading buffer (AB SCIEX) and were stored at -20°C until further processing.

Acidosis was neutralized by addition of 25 mM HEPES (Sigma-Aldrich Corp. St . Louis MO, USA) buffer so as to increase the buffering capacity of the culture media[ 10].

Following incubation of the sample for 2 hours at room temperature, the activity of the bactericidal agent was neutralized by adding an equal volume of 2% (v/v) Tween-80 (Sigma-Aldrich CheminaLambda UK) in Lambufferffer [ 15].

The formaldehyde was neutralized by the addition of 125 mM glycine and the cells were lysed with SDS lysis buffer.