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Accelerated degradation of the samples in an ethanol-containing pH 10 aqueous basic buffer was monitored in situ by digital imaging with a consumer-grade digital camera with simultaneous optical reflectance spectrophotometric point measurements.
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Apparent scattering of the solution at 360 nm due to heat induced aggregation of citrate synthase at 43°C at pH 7.9 (HEPES-KOH buffer), was monitored as a function of time in the absence and presence of αA- or αB-crystallins using a Jasco UV-visible spectrophotometer.
Fluorescence of the substrate in the reaction buffer was monitored for at least 5 min before the addition of the DNAzyme.
Release of lipase in pH 7.2 Tris buffer was monitored over 36 h using the micro BCA protein assay.
A single injection of 1 mM ATP (20 µL), prepared in the final dialysis buffer, was monitored for 3000 seconds.
The amount of released DOX in solutions of pH 7.4 (phosphate buffer) and 5.2 (acetate buffer) was monitored over time (up to 24 h) at an exciting wavelength of 480 nm (H in Figure 1).
The response of the microsensor for NO-oxidation was monitored in phosphate buffer using differential pulse voltammetry and constant-potential amperometry.
Binding of chemicals dissolved in the running buffer to immobilized Flag-SF-1 was monitored in real time by measuring changes in resonance units.
The in vitro release of DOX from micelles was monitored in phosphate buffer solution (PBS) 0.2 M (pH 7.4 and 5.5) containing 2% of Tween to evaluate drug release behavior in response to changes in pH.
After 1 h, 5 ng/μl trypsin or buffer alone was added and fluorescence was monitored in three or more replicates and control wells for the next 5 h.
Next, Cu release was monitored in 10 mM acetate buffer at pH 4, 5, and 6 containing a large excess of chloride (1 M NaCl, Figure 3D).
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