Sentence examples for buffer was monitored for from inspiring English sources

A single injection of 1 mM ATP (20 µL), prepared in the final dialysis buffer, was monitored for 3000 seconds.

Fluorescence of the substrate in the reaction buffer was monitored for at least 5 min before the addition of the DNAzyme.

Subsequently, the in situ resonance of a cantilever in buffer solution was monitored for every 20 minutes after injection of CTSB solution over 15 hours.

This sample was loaded on Sephadex G-75 (Amersham Biosciences, Sweden) gel filtration column (0.7 × 45 cm) which was preequilibrated with buffer A. The eluate was monitored for protein concentration at 280 nm and was assayed for fibrinolytic activity.

The enzyme reaction was started by adding substrate-buffer and the reaction was monitored for 60 min at 37°C as described above.

Each analyte (KD1L17R-VT and KD1L17R-KT, 100 2000 nM) was perfused through flow cells in HBS-P buffer (20 mM HEPES, pH 7.4, 100 mM NaCl, and 0.005% (v/v) P20) at 10 μL/min for 6 min. After changing to HBS-P buffer without the protein, analyte dissociation was monitored for 10 min. Flow cells were regenerated with HBS-P containing 20 mM εACA.

Then, the cells were lysed in lysis buffer, and 20 µl of each lysate was monitored for luciferase activity using Dual-Luciferase Reporter Assay System (Promega).

The test compounds diluted in assay buffer were then added, and fluorescence intensity was monitored for 150 s.

(d) The reaction of [1-C]GA catalyzed by 0.23 mM L6RM cTIM in the presence of 40 mM phosphite (50% dianion) in 6 mM imidazole buffer at I = 0.1 (NaCl) and 25 °C was monitored for 30 h, during which time the loss of 30% of the total of [1-C]GA was observed.

(b) The reaction catalyzed by 0.32 mM LDM cTIM in the presence of 40 mM phosphite (50% dianion) in 6 mM imidazole buffer at I = 0.1 (NaCl) and 25 °C was monitored for 140 h, during which time the loss of 80% of the total of [1-C]GA was observed.

(c) The reaction catalyzed by 0.39 mM L6RM cTIM in 20 mM imidazole buffer at I = 0.1 (NaCl) and 25 °C was monitored for 90 h, during which time the loss of 43% of the total of [1-C]GA was observed.