Sentence examples for buffer was mixed with from inspiring English sources
Briefly, 50 μl cell lysis buffer was mixed with 10 μl Ac-DEVD-pNA (2 mM) and 40 μl buffer and loaded into a 96-well plate.
In each run 0.1 mL of cell suspension (1 10 dilution in the resuspension buffer) was mixed with an equal amount of iso (baseline) or hyperosmotic solution (sorbitol or glycerol 2.1 M, 50 mM K-citrate buffer pH 5.1 or pH 7.4) of 1.25 tonicity ((Λ = (osmout)∞/(osmout)o)).
For enzyme extraction, 20 mL of the sodium acetate buffer was mixed with the solid medium (200 mM, pH 6.5).
For enzyme immobilization, 1 ml of lipase solution (1.0 mg ml−1 of lipase in 50 mM, pH 8.0 Tris HCl buffer) was mixed with 18 mg of NPG.
Briefly, 1 µl of 10× Fragmentation Buffer was mixed with 10 µl of DNA solution and incubated for 4 min at 95°C.
Chromatin in 100 µl RIPA ChIP buffer was mixed with 10 µl antibody-Dynabeads Protein A (Invitrogen) complexes overnight at 4°C.
Equal amounts of proteins (∼3 mg) in 600 µl of lysis buffer were mixed with an equal volume of 80% sucrose and laid at the bottom of ultracentrifuge tubes.
Extracts (500 µg protein/ml buffer) were mixed with 60% Optiprep™ (Nycomed, Denmark) to reach a final concentration of 40% and overlaid in a SW40 centrifugation tube (Beckman, CA, USA) with a step gradient of 30 and 5% Optiprep™ in MES-buffer.
Briefly, ~2.0 μg PCR products in QIAGEN EB buffer were mixed with an equal volume of 2x Binding and Wash buffer (1x buffer: 5 mM Tris, pH7.5, 0.5 mM EDTA, 1.0 M NaCl).
