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SDS-PAGE Sample Loading Buffer was mixed into the supernatant by proportion and the mixture was bathed for 5 min at boiling water.
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Tissue and buffer were mixed in a 1 20 relation.
Tissue and buffer were mixed in a 1 20 ratio.
Then, an additional 1.6 mL of buffer was mixed with the cells in the Petri dish and the cells were filtered through a 30-μm-mesh filter into a 5-mL cytometry tube.
To generate bead set pools, 3 μL of each oligo-bead conjugate was mixed into 1-mL 1.5× tetramethylammonium chloride (TMAC) buffer (Luminex Corporation).
The fungus was mixed into the diets.
Water-soluble compounds such as growth factors were mixed into the clotting buffer that was added on top of the polymerized gel (Fig. 1b).
Premix tubulin biotin streptavidin scintillation proximity assay beads (180 μl; 4.4 mg streptavidin yttrium silicate beads are mixed into 15 ml buffer and incubated on a slow 10 r.p.m. rotator at 4 °C for 30 min; Amersham Bioscience, Little Chalfont, Buckinghamshire, UK) were added to each well for 3 h at 37 °C.
1) Low Melting Agarose (LMA) - 1% LMA is mixed into the Phosphate buffered saline (PBS) and heat until the agarose dissolve in PBS.
The mRNA was mixed with the fragmentation buffer and fragmented into short fragments.
The mRNA was fragmented into short fragments when it was mixed with the fragmentation buffer.
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