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The release of reducing sugars in 60 min at 55°C and pH 4.8 (50 mM sodium citrate buffer) was measured as glucose equivalent using DNS method.
The serum concentration of hydroperoxides (whole oxidant capacity of serum against N,N-diethylparaphenylene-diamine in acidic buffer) was measured as described previously [ 27].
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Proteins (1 μmol/L) in Tris buffer were measured as previously described [ 17].
Titration of MT-DADMe-ImmA into enzyme-free buffer was measured and used as the control.
The pH of the buffer was measured after dialysis.
DNA concentration at two dilutions (1 100 and 1 1000 in TE buffer, pH 8) was measured as OD units at 260 nm and the number of plasmid copies in the extract was calculated from the molecular weight of the plasmid and the insert.
Twelve days after the start of differentiation, cells were lysed in GPDH buffer and GPDH activity was measured as described [71].
Immunoprecipitated (IP) HDAC2 was resuspended in HDAC assay buffer, and the activity was measured as indicated earlier.
DNA was resuspended in 120 μl TE buffer, and the concentration was measured as above and calculated to be 11.5 ng/μl.
The fluorescence intensity for the pseudoknot at 10 nM in 250 μL of standard melting buffer is measured and used as the starting point of the titration.
The gastric microsomes were then transferred to standard ATPase assay buffer at pH 7.4, and ATPase activity was measured as described above.
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