Sentence examples for buffer was measured after from inspiring English sources

The pH of the buffer was measured after dialysis.

Briefly, 3 μM F-actin (10% pyrenyl labeled) in actin polymerization buffer was measured alone or after the addition of either untagged or GFP-tagged cofilin (1 μM each) at 21°C.

The radioactivity associated with the cells and the incubation buffer was measured with a liquid scintillation counter after the addition of 3 ml of scintillation fluid (Clear-sol I; Nacalai Tesque, Kyoto, Japan) to the scintillation vials.

In brief, 100 μg of protein was incubated in a 96-well plate with 50 μM luciferin and 1.25 μg/mL luciferase in a Tris-based 1X reaction buffer containing DTT. ATP was measured after 5 min using a luminometer (560 nm at room temperature) using a standard curve of known ATP concentrations.

ABA was added to yeast cells suspended in 20 mM MES buffer (pH4.7) and ABACUS1 fluorescence was measured after 5 min. DxAm/DxDm values for yeast samples plus ABA were normalized to corresponding mock treated samples.

Telomerase (TERT) activity was measured after extraction of the proteins in CHAPS buffer (TRAPeze CHAPS Lysis buffer, Chemicon) as described [35].

Following 7 min of contraction in HK-buffer vessels were relaxed by washout using HEPES-buffered Krebs solution and the remaining tone was measured after 1 minute.

Total (T) actin content was measured after dilution of the samples with G actin stabilizing buffer (buffer A).

Total actin (T) content was measured after dilution of the samples with G-actin stabilizing buffer until maximal inhibition of DNase I was reached.

The total photoprotein content in the islets was measured after cell were lysed with a Triton X-100-based buffer.

Association of 500 nM [H]GDP was measured after 20 min pre-incubation of GDP-less membranes with buffer or carbachol.