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Non-enzymatic reduction of DHA in phosphate buffer was measured in a separate cuvette at the same time.
The release of reducing sugars in 60 min at 55°C and pH 4.8 (50 mM sodium citrate buffer) was measured as glucose equivalent using DNS method.
The pH of the buffer was measured after dialysis.
In comparison, the solubility limit of unmodified glucagon in the same buffer was measured at 0.06 mM (0.2 mg/mL), demonstrating that the XTEN sequence confers an increase in solubility of at least 60-fold.
Chemiluminescence generation by the reaction between superoxide generator and a luminous substance, MPEC, and its decay in the presence of the crude from a homogenized single larva with assay buffer was measured.
In the first case, after degranulation, the enzymatic activity of β-hexosaminidase in supernatants and cell pellets solubilized with 1% (v/v) Triton-X-100 in Tyrode's buffer was measured with p-nitrophenyl N-acetyl-β-D-glucosaminide in 0.05 M citric acid (pH 4.5) for 60 min at 37°C.
Similar(45)
The correlation between the two fields stored in the buffer is measured.
The insulin levels in conditioned KRB buffer were measured using a rat insulin RIA kit (Linco, St Charles, MO, USA) according to the manufacturers' instructions.
Proteins (1 μmol/L) in Tris buffer were measured as previously described [ 17].
The absorbance and elution volume of the protein complexes above the background signal of the buffer were measured at 260 and 280 nm (Table 1).
The fluorescence intensity for the pseudoknot at 10 nM in 250 μL of standard melting buffer is measured and used as the starting point of the titration.
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