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Exact(6)
To measure the optimum temperature, the pH of the experimental buffer was maintained at 4.5.
Whereas in the reconstruction of mTRPML1 in Amphipols A8 35, we did not add extra CaCl2 and the purification buffer was maintained at pH 7.4, which could not provide a sustaining Ca2+ inhibition on PMD.
The surrounding buffer was maintained at 37°C by a recirculation system, which has the combined benefit that the animal's body temperature is preserved.
The temperature of the buffer was maintained at 37°C using a circulating water bath.
The buffer was maintained at 37°C and continuously oxygenated with carbogen (95%O2/5%% CO2).
The final DMSO composition in the binding buffer was maintained at 2% v/v in the selectivity assays.
Similar(54)
Before the binding assays, all reagents and buffers were maintained at room temperature.
The buffers were maintained at 37°C by a heated water jacket and circulated by CO2/O2.
Buffer temperature was maintained at 37°C throughout the experiment.
The chamber was filled with 0.5% TBE buffer that was maintained at temperatures below 14 °C with tubing coiled in an ice bath.
The buffer gas was maintained at a pressure of ca. 4 Torr, and drift voltages were varied in order to correct for the non-IM flight time of ions through the interfacing ion optics.
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buffer was grown at
buffer was prepared at
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buffer was preheated at
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buffer was introduced at
buffer was stored at
buffer was conducted at
buffer was nucleated at
buffer was preincubated at
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