Sentence examples for buffer was kept at from inspiring English sources

The buffer was kept at 4 °C throughout the experiment to prevent capping of antibodies on the cell surface and non-specific cell labelling.

The buffer was kept at 29°C and bubbled continuously with 95%O2/5%% CO2.

The agar pad and M9 buffer were kept at the initial imaging temperature.

All buffers were kept at 37 °C during imaging using a water bath.

All the equipment and buffers were kept at 4°C before the extraction, and all the steps were conducted on ice or at 4°C.

The tissue segments were constantly aerated with a mixture of 95% oxygen and 5% of carbon dioxide, and the buffers were kept at 37°C using an external water bath.

All buffers are kept at 4°C and the protease inhibitor cocktail is diluted fresh from frozen stocks (200 mM PMSF in isopropanol [kept at room temperature], 1 M Benzamidine in H2O, 3 mg/ml TPCK in ethanol, 1 mg/ml leupeptin in H2O, 1 mg/ml pepstatin in methanol, 2 mg/ml aprotinin in 0.1% NaCl and 1M DTT in 10 mM NaAc pH 6).

The catalytic activity of Ad-APR-1, Ay-APR-1, and Ac-APR-1 was similarly determined, except that the buffer was kept constant at pH 3.5.

The flow rate of the low-pH buffer was kept constant at 0.075 ml/min for the duration of imaging, except in a single experiment (Video 2), where the flow was stopped to allow switching from low-pH back to neutral-pH buffer flow.

The buffer temperature was kept at 14°C during the PFGE run.

The artery segments were immersed in buffer solution which was kept at 37° C and continuously aerated with a gas mixture containing 95% O2 and 5% CO2 with a pH of 7.4.