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After the 2 h of reperfusion, 40 ml of 1% (w/v) solution of triphenyl tetrazolium chloride (TTC) in phosphate buffer was infused into aortic cannula, and the heart samples were stored at -70°C for subsequent analysis.
To control for the diluent needed to solubilize lolitrem B and paxilline, 0.1% DMSO in Krebs-Henseleit buffer was infused into the aortic cannula for 12 min, followed by 30 min monitoring of cardiac activity.
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Biotinylated F1 (500 pM) in buffer A was infused into an observation chamber which was constructed of two coverslips: a bottom one (24 × 32 mm) modified with Ni-NTA (9) and an untreated top one (18 × 18 mm).
TNBS solution (0.5 ml, 2%) in 10% ethanol in phosphate buffered saline (PBS, pH 7.4) was infused into the pancreatic duct over a period of 2 5 min. The hole in the duodenum was sutured and the vascular clamp was removed restoring the bile flow.
0.1 ml of 1% TNBS in phosphate buffered saline (PBS -10% ethanol, PBS -10as infused into the pancreas (modified after Puig-Divi [ 13]).
After transferred to 50 mM ammonium acetate buffer pH 5.0 a 1% carboxymethylcellulose (CMC) solution was infused into the SDS-free polyacrylamide gel, incubated at 37°C for 120 min, and stained with Congo red [ 65- 68].
These effects were eliminated when muscimol was infused into dmPFC.
"In fact, no money was infused into the institution".
The liquid material, EVAL-g-PnF dissolved in DMSO, was infused into an aneurysm model.
Between T = 0-120min 20% intralipid was infused into the duodenum at 2kcal/min.
CO2 was infused into respiratory lung models to reach PETCO2 of 40 mmHg without HFNC.
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