Sentence examples for buffer was incubated with from inspiring English sources

To label transferrin protein, 1 nmol of Tf (apo-transferrin, Sigma-Aldrich) dissolved in reaction buffer was incubated with 10-fold molar excess of AF488-NHS ester in DPBS at room temperature for 2 h.

Next, 100 µL of 0.1% ChonBlock™ blocking buffer was incubated with the probe antibody-conjugated microbeads with vortexing at 1400 rpm for 2 h at room temperature.

Reactions to determine the glycosyltransferase activity of MrSGT on fucosterol and gramisterol, were performed as follows; MrSGT dissolved in the above-mentioned reaction buffer was incubated with 0.6 mM each of fucosterol and gramisterol, respectively, along with 0.8 mM of UDP-Glc at 30 °C for 15 min, and then extracted by organic solvent partition.

250 µl 80% human CSF in capture buffer was incubated with 30 µl ASR1 beads.

LC/MS: Lung homogenate (0.25 0.5 µg) in homogenation buffer was incubated with GSNO (28 µM) and 2 mM GSH in the presence or absence of 300 µM NADH at 37°C for 5 minutes.

0.5 µg of purified 26S proteasome (Biomol, Plymouth Meeting, PA) in 10 µl lysis buffer was incubated with and without 50 µM epoxomicin (Sigma Aldrich) for 1 hr at 4°C, and used as negative and positive control, respectively.

Suspensions of washed human platelets (2×10 cells mL−1) in Tyrode's buffer were incubated with different carrier Lifeact systems (concentrations of incubation between 0.5 and 10 μ m were investigated), transferred onto cover slips for spreading on fibrinogen and fixed.

Cells harvested in the above mentioned lysis buffer were incubated with the primary antibody overnight at 4 °C which was followed by an incubation of 90 min with protein A or protein G sepharose beads (GE Healthcare, Uppsala, Sweden).

Secondary antibodies (Life Technologies) in blocking buffer were incubated with cells for 2 hours at room temperature in the dark, when they were washed 3 times in PBS with 0.1% Triton X-100.

To prepare 25%CLox LUV, 25%CL LUV in EDTA-free KHE buffer were incubated with 20 μM CuCl2 at 37°C, and CL oxidation was checked by monitoring absorbance at 245 nm in a Uvikon 922 spectrophotometer (Kontron instruments).

50 μl (≈ 106) protoplasts, re-suspended in transformation buffer, were incubated with 10-20 μg of plasmid DNA for 20 min at 4°C. 100 μl of 24% (w/v) PEG 6000 was added, gently mixed and incubated for 30 min at room temperature.