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Sample buffer was included as a blank, its value being subtracted from all samples (measured in triplicate).
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MRB buffer only (in HBSS) was included as a negative control.
A contingency buffer is included in the start-up cost to ensure the business in not under financed, as well as giving the business adequate funding to sustain it in the first six months of start-up.
Samples of the lysis buffer only were included as a negative control for the RT-PCR and sample of lysis buffer supplemented with total RNA extracted from HNSCC cell line monolayers were used as a positive control for the RT-PCR.
A volume of 100 μl of 1% Triton X-100 and buffer alone were included as controls for 100% release and for spontaneous release, respectively.
Phase 1 and 2 is independent of phase 3. The same buffer-map scheme is included as described in the conventional push approach above [45 47].
were included as outgroups.
CL-Pgp, WT and selected Cys mutants were purified by affinity and SEC as described, except that NaCl (250 mM) was included in all buffers, as higher salt concentration have been shown to improve purification of human Pgp (see the Materials and Methods section for details) [ 30, 42].
DFHBI-1T (20 µM) was included in cell-binding buffer when 3WJdB was used as payload.
DFHBI-1T (20 µM) was included in cell-binding buffer only when 3WJdB was used as payload.
Depending on local conditions, drought-resistant varieties or buffer strips could be included.
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