Sentence examples for buffer was generated by from inspiring English sources

The buffer was generated by assuming that the plot was surrounded by similar plots on all sides.

In short, RNA was generated by lysing cells in RNAeasy buffer (Qiagen) and then following the standard RNAeasy protocol.

After the geometric buffer is generated, we first use the bilateral buffer on its depth buffer to obtain a smooth surface, and then, we integrate foreground and background scenes by background mapping.

Apopolypeptide was generated by dialysis of native polypeptide against low-pH buffer under reducing conditions.

Double stranded DNA was generated by mixing equimolar amounts of the complementary oligonucleotides in annealing buffer (Ambion, CA, USA).

A cell pellet was generated by centrifugation and the recombinant protein was solubilized using a buffer containing non-ionic detergent.

A standard curve was generated by preparing a stock of 10 mM NaHPO4 in assay buffer which was stored at -20°C when not in use.

The ACDha variant was generated by the cysteine to dehydroalanine reaction as described above and then exchanged into gel filtration buffer without BME.

RNase T1 ladder was generated by incubating 0.2 pmol of denatured RNA with 0.1 units of RNase T1 in 1× sequencing buffer (Ambion) for 5 min at 37°C.

The SaeP antibody was generated by Genscript.

Contrary, an inside negative membrane potential was generated by using 50 mM sucrose, 100 mM Kgluconate, 0.1 mM Ca(gluconate 2, 20 mM Hepes/Tris pH 7.4 as resuspension buffer (Kout < Kin resuspension buffer) and 250 mM sucrose, 0.1 mM Ca(gluconate 2, 2.5 mM Mg(gluconate 2, 20 mM Hepes/Tris, pH 7.4 as incubation buffer (Kout < Kin incubation buffer).