Sentence examples for buffer was frozen at from inspiring English sources

A nonincubated raw lysate control containing 800 μg protein in 400 μl SAP buffer was frozen at -80°C without additions or incubation.

After centrifugation and one wash step with PBD-/GTP γS-PBD-buffer, the pellet was frozen at −80 °C.

Aliquots of 150 µl worms in M9 buffer were frozen in liquid nitrogen and stored at −80°C.

The pellet was then homogenized in 50 mM Hepes, pH 8.0 containing 5 M urea and centrifuged at 150,000× g for 45 min. Membranes were washed three times with Ringer's buffer, and pellet containing urea washed ROS was frozen at −80°C for further use.

Each sample collected was washed twice with phosphate buffered saline (PBS) and the cell pellet was frozen at −80 °C until sample processing.

The enzyme was frozen at −20°C in the same buffer containing 20% glycerol.

The platelet pellet was frozen at -80°C in citrate wash buffer to minimize in vitro platelet activation.

Concentrated RIPA lysis buffer was added to the supernatant, homogenate was incubated on ice for 15 30 minutes, spun at 10,000 × g for 10 minutes, and parenchymal supernatant was frozen at −80 °C until further processing.

Blood Plasma was frozen at −70 °C.

For RNA analysis the cells were lysed in Qiazol buffer and were frozen at -80°C.

After these treatments, cells were treated with Qiagen RLT buffer and were frozen at −80°C before RNA extraction.