Sentence examples for buffer was fractionated through from inspiring English sources

A quantity of 0.55 ml of soluble nucleosome containing buffer was fractionated through a sucrose density gradient solution (5%to25%5% sucrose, 10 mM Tris-HCl, pH 7.4, 0.25 mM EDTA) containing NaCl of indicated concentrations, by centrifuging at 30,000 rpm for 16 h at 4°C.

The reaction, stopped by the addition of Laemmli sample buffer, was fractionated on 10% SDS-PAGE and subsequently silver stained as described [39].

The fulvic fraction (acid soluble) was fractionated through a column of Amberlite XAD-7 resin.

To purify small RNA, total RNA was fractionated through a flashPAGE Fractionator (Applied Biosystems), and small RNA was retrieved.

Samples containing 50 75 µg of protein in reducing sample buffer were fractionated by sodium dodecyl sulfate polyacrylamide gel electrophoresis.

Flagellar matrix was fractionated through a 10 25% sucrose density gradient.

The residue was fractionated through silanised silica, eluting with a gradient of water CH3CN, plus sat.

The polysaccharides were fractionated through ion-exchange chromatography, and were shown to be homogeneous via high-performance size-exclusion chromatography (HPSEC).

GST fusion proteins were fractionated through 10% SDS-PAGE before their immobilization to nitrocellulose membranes.

NEs prepared from uninfected cells were fractionated through a phosphocellulose column by stepwise elution with increasing concentrations of KCl.

RNA was fractionated by electrophoresis through a 1.2% agarose gel and visualized by ethidium bromide staining.