Sentence examples for buffer was followed by from inspiring English sources

Dilution of 1 μl of serum in phosphate-buffered saline/0.3% bovine serum albumin (pro analysi buffer) was followed by overnight incubation with 1 mg of Sepharose-immobilized protein A (GE Healthcare, Chalfont St Giles, UK) in a final volume of 800 μl.

For immunohistochemistry heat antigen retrieval in citrate buffer was followed by washing the sections in aqua dest.

Thereafter, a rinse with phosphate buffer was followed by staining with Cobalt enhanced DAB-Ni substrate: 0.025 g DAB, 1 ml 1% nickel-ammonium sulfate, 1.25 ml 1% Cobalt chloride, 20 μl of 30%H2O22 in 50 ml PB, for 10 30 min at rt.

The sections were then incubated in primary antibody diluted in blocking buffer overnight in cold room (4 °C), followed by three times rinse in PBS-T and 1 h incubation in secondary antibody in blocking buffer was followed by three times rinses in PBS-T.

Application of cell lysate in binding buffer was followed by a washing step with 10 ml of binding buffer containing 60 mM imidazole and an elution step with 5 ml of elution buffer (50 mM Hepes/NaOH, pH 8.0, 300 mM NaCl and 500 mM imidazole).

Thereafter, washing in 1× PermWash buffer was followed by a 45-min incubation period at RT in 100 μL of a secondary solution containing 1 200 rabbit anti-mouse AlexaFlour488-conjugated Ab (Abcam) and 1 50 TO-PRO solution (Invitrogen, Carlsbad, CA, USA) in 1× PermWash buffer.

Three 60 s start-up injections of Nanodisc SPR buffer were followed by serial injections of nanodiscs for 90 120 s.

Extensive washing with streptavidin binding buffer (SBB) was followed by 30 min incubation with 2 mM biotin at 4°C.

The beads were washed and resuspended in SDS sample buffer; this was followed by an immunoblot analysis.

The cleaved sample was eluted by adding 500 μl of TEV cleaving buffer and was followed by a calmodulin binding step [18].

Each individual stage was followed by buffer rinses (EnVision FLEX wash buffer; Dako).