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The buffer was filtered through 0.45 μm filter before it was transferred to the inlet/outlet vials.
The sodium dihydrogen phosphate buffer was filtered through filters of 0.02-µm pore size (Whatman, Dassel, Germany) before use.
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Following washing twice with ice-cold PBS, the cell suspensions in 800 μl FACS buffer were filtered through a 70 mm nylon mesh.
Finally, the stained cells (1 ml in flow buffer) were filtered through 40 μm nylon cell strainers from BD Biosciences (Bedford, MA, USA) and analysed on a FACSCalibur cytometer (Becton Dickinson, San Jose, CA, USA) at the London Regional Flow Cytometry Facility Robarts Research Institutee, London, ON, Canada).
All buffers were filtered through sterile 0.22 µm filter before use.
All the incubations were performed under sterile conditions (i.e., all glassware and cuvettes were sterilized and all buffers were filtered through 0.02 µm Whatman Anotop filters).
All buffers were filtered through 0.22 μm filters.
All buffers were filtered through a 0.45-μm filter (Millipore, Tokyo, Japan) before the experiments.
Buffers were filtered through a 0.22-μm filter before usage to remove any PK9119 precipitates.
All buffers were filtered through a Whatman syringe filter (0.20 μm pore size, Whatman Plc).
All samples and buffers were filtered through a 0.2 μm filter (Millipore) before analysis.
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