Sentence examples for buffer was exchanged with from inspiring English sources
The extraliposomal buffer was exchanged with 10-mM HEPES-buffered saline (HBS, pH 6.5) via tangential flow filtration (Spectrum Labs; Rancho Dominguez, CA, USA).
Finally, pure and homogenous fractions were collected and buffer was exchanged with a buffer containing 20 mM KPi (pH 7.6), 100 mM NaCl and 0.15% DDM.
The protein buffer was exchanged with 10 mM phosphate buffer, pH 7.4, containing 150 mM NaCl, using the centrifugal ultrafiltration filter.
Before deAMPylation reactions, the buffer was exchanged with 50 mM Tris-HCl pH 8.0 and 150 mM NaCl by 5 cycles of dilution/concentration on a Vivaspin-500 with a cut-off of 50 kDa (Sartorius), resulting in a final dilution of ATP-FAM, MgCl2, and PPi produced by the reaction of about 105 times.
The buffer was exchanged with PBS by using a PD-10 column (GE Healthcare), and the purified protein was stored at −80°C until use.
Supernatants containing the recombinant protein were concentrated and the buffer was exchanged with PBS, 250 mM NaCl using an Amicon Centrifugal filter.
Free dye was then excluded by passing the protein solution through a PD-10 column and buffer was exchanged to PBS with 0.1 M NaHCO3 at pH 8.5.
Human recombinant proMMPs and in vitro activated MMPs were obtained from Invitek [ 44] and were labeled with the [I]-Bolton-Hunter reagent according to the manufacturer's instructions (PerkinElmer, Rodgau, Germany) before the buffer was exchanged for phosphate-buffered saline (PBS) with 0.05% (vol/vol) Tween 20 by gel filtration as described previously [ 45].
Proteins were concentrated, and buffer was exchanged to wash buffer with 10% glycerol by using an Amicon centrifugal filter device (Millipore).
Buffer A was exchanged with buffer B by several concentration/dilution cycles using a Vivaspin sample concentrator.
For near-infrared (NIR) MCD measurements, the protein buffer solution was exchanged with a deuterium oxide buffer solution of 100 mM MOPS buffer (with100 mM NaCl) at pD = 7.0 until D2O comprised more than 99.5% of the solvent.
