Sentence examples for buffer was exchanged by from inspiring English sources

Buffer was exchanged by addition of 0.1 M Tris pH 7.5, 1 mM NaCl, 5 mM DTT while concentrating the protein by ultrafiltration.

Buffer was exchanged by gel filtration on Sephadex G-25 column (Amersham) with 50 mM TRIS at pH 7.5, 150 mM NaCl at 4°C.

Before crystallisation or limited proteolysis assays the buffer was exchanged by cycles of concentration and dilution in 0.04% C12M, 20 mM Tris-HCl pH 8, 0.1 M NaCl on an Amicon concentrator equipped with a 50 kDa cut-off membrane.

After AXH purification, the buffer was exchanged by dialysis to 20 mM Tris HCl (pH 7.0), with 20 mM NaCl, 2 mM β-mercaphtoethanol and 0.01% (wt/vol) NaN3 and then concentrated to a final concentration of 0.3 mg/ml.

For both scaffoldin and cellulases the buffer was exchanged by dialysis against TBS, and the scaffoldin sample was concentrated using Amicon Ultra 15 ml 50,000 MWCO concentrators (Millipore, Bedford, MA, USA).

The fractions containing MTS(L)-Cyt f were concentrated and the buffer was exchanged by ultrafiltration to MES (20 m m, pH 6), K3Fe CN 6 (0.5 m m).

The buffer was exchanged with PBS by using a PD-10 column (GE Healthcare), and the purified protein was stored at −80°C until use.

Fractions of the eluate containing the purified protein were pooled and the buffer was exchanged to PBS by dialysis.

Proteins were concentrated, and buffer was exchanged to wash buffer with 10% glycerol by using an Amicon centrifugal filter device (Millipore).

For crystallization, the buffer was exchanged to 10 mM Tris-HCl pH 8 by diluting and re-concentrating the enzyme in 2 mL Ultra Centrifugal Filters (Amicon).

Thymic fragments were pelleted by centrifugation at 300× g and the digestion buffer was exchanged.