Sentence examples for buffer was exchanged at from inspiring English sources

To compensate for evaporation, the buffer was exchanged at regular intervals of 1 h.

The entire volume of 1X PBS buffer was exchanged at 1, 3, and 5 days.

Buffer was exchanged at time points of 8, 15, 30, 60, and 120 min. The recovered solution's protein concentration was evaluated using a Micro BCA protein assay (Thermo Scientific, Rockford, IL).

The lipid films were hydrated with ammonium sulfate buffer pH 5.5 at 65°C, the resulting liposomes extruded through a 100 nm pore size filter, and next the buffer was exchanged to citrate buffer at the same pH to allow for doxorubicin loading.

In this period, the buffer was exchanged 10 times after at least 30 min. After renaturation, the gel was incubated 2 times for 30 min, respectively, in kinase buffer (20 mM HEPES, 20 mM MgCl2, 50 µM CaCl2 or 500 µM EGTA, 1 mM DTT, 0.05% Tween 20, pH 7.5), followed by 30 min incubation in kinase reaction solution (kinase buffer containing 50 µM ATP and 100 µCi γ-P-labeled ATP).

Free dye was then excluded by passing the protein solution through a PD-10 column and buffer was exchanged to PBS with 0.1 M NaHCO3 at pH 8.5.

The dialysis buffer was exchanged daily and the sample was incubated at 27°C for 10 to 21 days.

Afterwards, the buffer was exchanged to a 50 mM glycine buffer at pH 10 to stop the protease activity and to prepare for cation exchange chromatography.

At the same time the buffer was exchanged with MQ water.

Thymic fragments were pelleted by centrifugation at 300× g and the digestion buffer was exchanged.

The buffer was exchanged to 10mM sodium-phosphate (pH 7.0) and the SPRR proteins were stored at −80°C.