Sentence examples for buffer was done in from inspiring English sources

To demonstrate the improvement in microbial oil accumulation of activated sludge a fed-batch feeding of acetic acid via acetic acid-sodium acetate buffer was done in 5-L bioreactor.

Lysis in the P2 buffer was done for 15 min at room temperature and 15 min at 65°C.

A control in phosphate or acetate buffer without additives was done in parallel.

Primary antibody staining was performed using above-mentioned buffer for ~8 hrs at RT or ~ 16h at 4 degrees followed by three washes of 15 minutes in blocking buffer at RT. Secondary antibody staining was done in the blocking buffer for 1-2 hours and washed three times with blocking buffer for 15 minutes followed by DAPI staining for 30 minutes and 2 additional washes in blocking buffer.

The final dialysis was done in buffer containing 50 mM Urea.

DNA labeled at 5′ end using 6-mercapto-1-hexhane (HS-ssDNA) was immobilized on AgNPs-Pdop@Gr surface to form ssDNA-S-AgNPs-Pdop@Gr and hybridization sensing was done in phosphate buffer.

The particle concentration for zeta potential measurements was 330 μg/mL in 0.001 M phosphate buffer (pH 7.4), and the measurement was done in triplicate with over 13 sub-runs.

The particle concentration for hydrodynamic diameter measurements was 30 μg/mL in 0.001 M phosphate buffer (pH 7.4), and the measurement was done in triplicate with over 17 sub-runs.

In vitro CC release from SSNEDDS-5 formula was done in phosphate buffer (pH 6.8) containing 0.35% of tween 20 and in water with 2% of tween 20 (Fig. 6) and it has shown that release of CC was faster in phosphate buffer (pH 6.8) and lower in water compared with their release in 0.1 N HCl medium (pH 1.2).

Staining was done in labeling buffer (PBS plus 5% FBS).

Elution was done in elution buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA pH 8.0, 1% SDS) for 15 min at 65°C.