Sentence examples for buffer was dissolved in from inspiring English sources

HEPES buffer was dissolved in 99.8% D2O and lyophilized, twice, to produce deuterated HEPES (D-HEPES).

The first part of the above protein pellets (1 mg RJ/100 μl buffer) was dissolved in 40 mM of (NH4 HCO3 (Sigma).

Rat tissue extracts (2.5 mg, prepared in IEF lysis buffer) were dissolved in a final IEF buffer volume of 2.5 ml, applied to MicroRotofor (Bio-Rad), and electrophoresed for 1.5 2.5 hours (1W constant power, 4°C).

Cell pellets washed with 10 ml MACS buffer were dissolved in 500 μl of MACS buffer.

Then, each membrane was blocked with BSA (bovine serum albumin, Sigma, Germany) at room temperature for 1 h (in the blocking buffer, BSA was dissolved in 1 × TBS- 0.1% Tween to a final concentration 0.02 g/mL).

For the Z-sarcosyl-buffer, 0.5% N-lauroyl-sarcosine was dissolved in LacZ-buffer with 2mM DTT.

The precipitate was dissolved in buffer B and the resulting solution dialysed against this buffer.

The LA was dissolved in buffer and continuously infused into the fermenters.

Purified Burkholderia cepacia lipase (366 U) was dissolved in buffer and ultrasonicated at 110W.

The new pellet was dissolved in buffer A (100 mM NaCl, and 0.4 % v/v TRITON X-100) and centrifuged.

Free GOx was dissolved in buffer (200 mM, pH 4.0 8.0); 0.2 mL free enzyme (100 U/mL) was added into solutions.