Sentence examples for buffer was discarded by from inspiring English sources

After 10 minutes the assay buffer was discarded by vacuum aspiration and 25 µl of assay diluent was added to the wells designated for the samples, while 25 µl of RPMI-1640 with glutamax (GIBCO) was added to the wells designated for standards.

The blocking buffer was discarded, and the primary antibody preparation (rabbit anti-CBM, diluted 1 3000 in blocking buffer) was added.

The supernatant was discarded by inversion and the pellet was suspended in remaining buffer by vortexing.

This means that each time a bundle is forwarded to a node, the sender node keeps a copy of this bundle on its buffer until the bundle is discarded by buffer congestion.

In packet-based networks packet losses may occur, either because of packets which do not arrive at the listener's side or because of packets which have to be discarded by the receiving buffer management algorithm due to real-time transmission constraints.

Such areas are discarded by extracting the results lying only within a buffer of 400 m on each side of highways.

Eight defining characteristics were discarded by the patients.

Tissues were thawed on ice, and the original buffer solution was discarded.

The supernatant was discarded followed by peptide dilution in 50 μl of loading buffer.

Then, the BSA buffer in each well was discarded and the plate was washed three times with PBST buffer (0.05% Tween-20 in PBS buffer).

Samples were washed in binding buffer, and the supernatant was discarded.