Sentence examples for buffer was diluted with from inspiring English sources

The lumen fraction was concentrated using Microsep 3K centrifugal devices (PALL Life Ccience) and the buffer was diluted with double distilled water approximately to half the strength in order to decrease the salt concentration prior to iso-electric focusing (IEF).

The 3.5% hyaluronan thiomer gel (HA gel) produced and provided by Croma Pharma GmbH (Leobendorf, Austria) in 80 mM phosphate buffer was diluted prior the surgical procedure with a buffer containing 0.06% H2O2 and 120 mM phosphate buffer.

Then the plasmid DNA stock in TE buffer (5 8 mg/ml) was diluted with 5% glucose solution to a final concentration of 1 µg DNA/µl volume.

Briefly, freshly thawed serum was diluted with dilution buffer and added to each well.

The clarified supernatant was diluted with dilution buffer (1 1 ratio) and 5 μl of diluted samples were removed as an internal control.

The IREF-2 activity fraction eluted with buffer H containing 0.6 M KCl was diluted with buffer H and again loaded onto the Uno-Q column equilibrated with buffer H containing 0.25 M KCl.

A mixture of unmodified and seco-modified peptides in 10 mM NH4HCO3 buffer (pH 7.4,100 μL) was diluted with chloroform and methanol in a ratio of 8 4 3 chloroform/methanol/buffer.

Purified protein sample was diluted with buffer to 400 μL with an A280 absorption of about 0.7.

Total protein lysate (15 mg) was diluted with buffer A (20 mM KPO4 pH 8.0) to 4.5 ml.

This nanoparticles solution was diluted with buffer and used as such for the performing the degradation experiments using Nile Red.

The affinity purified sample was diluted with Buffer A 1 5 for negative stain analysis and 1 3 for cryo-EM analysis.