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The strain related to a reference zone chosen in the GaAs buffer was determined with a spatial resolution better than 1 nm, by means of geometrical phase analysis of the HR-TEM images [13, 14].
The feces of challenged mice were collected prior to challenge (day 0) and daily for 8 d after the challenge, and the presence of RV antigen in fecal samples suspended in TNC buffer was determined with ELISA as previously described.
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Salivary mutans streptococci and buffer capacity was determined with Dentocult® SM - Strip mutans and Dentobuff® Strip, respectively.
The yield of sorted nuclei in sort buffer (PBS) was determined with a hemacytometer, and 50 200 K nuclei were used for each ChIP.
Recently, the number of P. ostreatus laccase isoenzymes in buffered and non-buffered media was determined with the initial pH adjusted to 3.5 in both culture media.
Furthermore, total protein was extracted from lumbar CLC-rich NPs from non-chondrodystrophic and chondrodystrophic dogs using radioimmunoprecipitation assay (RIPA) buffer; protein concentration was determined with a Lowry assay.
The integrity of the total RNA was checked by electrophoresis in 1% agarose gel in Tris-acetate-EDTA (TAE) buffer and quantity was determined with a NanoVue™ Spectrophotometer (GE healthcare).
The resulting pellets were homogenized in 500 ul of buffer and protein concentration was determined with a BCA protein assay.
Briefly, cellular protein was extracted from mouse liver using lysis buffer, and protein concentration was determined with a BCA protein assay kit (KeyGEN, China).
Unbound iron and sulfide was removed by desalting on a 10 ml Sephadex G-25 column (GE Healthcare) equilibrated in buffer A. Protein concentration was determined with the Microbiuret method at 545 nm [ 36].
Enzymes peroxidase, lactoperoxidase and alkaline phosphatase in phosphate buffer were tested and activity was determined with commercial reflectometric strips.
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