The resulting amino-allyl labeled DNA was coupled with Cy dye (GE Healthcare), cleaned, and hybridized using SlideHyb #1 hybridization buffer (Ambion).
Purified cDNA was coupled with N-hydroxysuccinimide esters of Cy3 or Cy5 (GE Lifesciences, Baie d'Urfe QC) in bicarbonate buffer, following the manufacturer's instructions.
Furthermore, the concentration of the buffer could also have negative effects on crystallization, especially in the case of salt containing buffers when increasing the concentration is coupled with an increase in ionic strength.
Carboxylated poly methyl methacrylate) microbeads (PolyAn GmbH, Berlin, Germany) were coupled with recombinant His-PA28γ following MES buffer method [ 36].
Carboxy-coated microspheres were coupled with capture antibody, using the appropriate coupling buffers.
The reactor was coupled to a buffer with a free volume of roughly 200 mL.
Briefly, about 50 RU recombinant MUC1 15 aa with cysteine peptide (APDTRPAPGSTAPPA-C) were coupled in 10 mM sodium acetate buffer pH 4.0 on a CM5 chip after activation with NHS/EDC chemistry, followed by PDEA in 80 mM borate buffer.
The proposed buffer consists of six microring array coupled via upper MZI arm, and one microring is coupled via the lower MZI arm, where all coupling gap used is with 105 nm.
Immunocomplexes were coupled to protein G-coated Dynabeads (Invitrogen) for 1 h, washed with lysis buffer and used directly in in vitro methylation studies.
However, we also show that ΔAB<0 (conduction band lower in the buffer than in the absorber) is usually coupled with an efficiency loss that is even worse if ΔBW>0.
