Sentence examples for buffer was conjugated with from inspiring English sources
Methods: Trastuzumab, dialysed against 50 mM EDTA, washed and recovered in metal-free HEPES buffer, was conjugated with tripodal tris(hydroxypyridinone) (THP) (3.23 mg trastuzumab, 0.8 mM H3THP-Ph-SCN; 260 μL) and purified by size exclusion chromatography (SEC).
For fluorescent nanoformulations, Alexa Fluor® 488 carboxylic acid succinimidyl ester was conjugated with IgG SPIO in carbonate-bicarbonate buffer for 2 h.
The secondary antibody (Jackson ImmunoResearch Laboratories) was conjugated with horseradish peroxidase at an appropriate dilution by blocking buffer.
Briefly, dyes were conjugated with proteins in a sodium phosphate buffer (100 mM, 0.15 M NaCl, pH 7.2) and unconjugated dyes were neutralized by ammonium chloride.
The antibodies were conjugated with p-SCN-Bn-DOTA at pH 9 (sodium borate buffer) at 37°C over night.
CNB-activated Sepharose 4B (GE-Healthcare, Uppsala, SE) was equilibrated in buffer solutions, conjugated with capture antibodies according with manufacturer's instructions and packed in 2 mL or 5 mL Bio-Scale MT columns (BIO-RAD).
The buffer was then replaced with ATP assay buffer.
The standards of 25, 50, 100, 250, and 500 ng/ml, undiluted rat heparin plasma, and buffer were incubated together with zonulin HRP conjugate on a plate pre-coated with monoclonal anti-zonulin antibodies.
Three washes with wash buffer were needed before incubating for 1 h each with anti-CML antibody and with secondary antibody-HRP conjugate.
