Sentence examples for buffer was conducted at from inspiring English sources

The enzymatic hydrolysis of cellulose (Sigmacell cellulose type 101) with 40 g/L in 0.05 M citrate buffer was conducted at pH 4.8, 50°C and 200 rpm.

For this, recombinant baculovirus encoding yeast Rce1 cDNA was infected to Spodoptera frugiperda (Sf9) insect cells to express recombinant Rce1, and 200 μg of the Sf9 membranes was added to POG-Tβγ-VIS in 10 mL reaction mixture in buffer P. The proteolytic reaction was conducted at 37 °C for 2 h.

Sf9 insect cells were infected with recombinant baculovirus encoding yeast Icmt cDNA to express recombinant Icmt, and 400 μg of the Sf9 membranes and 10 μM final concentration of AdoMet were added to POG-Tβγ in the 10 mL reaction mixture in buffer C. The carboxyl methylation reaction was conducted at 30 °C for 3 h.

The assay was conducted at 30°C in PBS buffer.

Each assay was conducted at 21°C in collagenase assay buffer.

Electrophoresis was conducted at 4 °C in Tris glycine SDS running buffer according to the standard running conditions.

Electrophoresis was conducted at 100 V in TBE 0.5 × buffer.

Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) (Laemmli, 1970) was conducted at constant wattage (10 W) in running buffer cooled to 4°C.

Electrophoresis was conducted at pH 6.5, and pH 8.0 in a buffer puffer (Owl Separation Systems) recirculating electrophoresis box in order to maintain a uniform pH.

Enzymatic hydrolysis was conducted at 2% substrate solids (w/v) in pH 4.8 buffer solution on a shaker/incubator at 50°C and 200 rpm.

Staining was conducted at 4°C in several sequential steps in FACS staining buffer (1X PBS with 2% FCS, 0.1% NaN3), washing twice between steps.