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The sample buffer was complemented with β-mercaptoethanol to a final concentration of 1% and samples were heated to 70°C for 10 min.
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The experimental study was complemented with computational framed models.
Also, the mutant JG27 was complemented with cloned SHJG0322.
Hence, plantation of riparian buffers should be complemented with additional mitigation measures such as improving the groundwater table recharge, managing the temperature of urban discharges, to mitigate climate change impacts on ST.
Table 2 describes the behavior of scenario A, where the network and buffer status can be complemented with other application layer parameters as inputs to the DASH algorithm.
Left hemispheres were complemented with lysis buffer containing 25 mmol/l HEPES, pH 7.5, 1 mM EDTA, 10 mM 1,4-dithiothreitol (Roche, Germany), 0.1% 3-[ 3-cholamidopropyl dimethylammonio]-1-propanesulfonate (CHAPS; Sigma-Aldrich, Germany), 10% sucrose, 0.1% Triton X-100 with 10 μg/ml of pepstatin A, aprotinin and leupeptin plus 1 mM phenylmethylsulfonyl fluoride (PMSF; all Sigma-Aldrich, Germany).
All buffers used for vesicle generation, isolation, and analysis were complemented with 0.2% BSA and filtered (0.22 μM) before use.
"They must be complemented with other reforms".
But our commitments need to be complemented with implementation.
The interviews were complemented with phone discussions.
This system is complemented with Eqs.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com