Sentence examples for buffer was collected as from inspiring English sources

After the treatment, the buffer was collected as the extracellular fraction.

An additional 3.5 ml of PBS was added to the column, and the displaced buffer was collected as the elution fraction.

The remaining infiltration buffer was collected as "supernatant".

Following incubation, 50 μl of preheated 95°C hot SDS gel loading buffer was added and elute was collected as immunoprecipitate.

From each site, the tissue was collected as fresh frozen and also in RNA-stabilizing buffer.

The 100,000× g supernatant was collected as the S3 fraction and the pellet was resuspended in 40 70 µl of Buffer A. P2 was washed twice by resuspending cells in 100 µl Buffer A and pelleting (10,000× g for 15 min).

Serial coronal sections (50 µm) containing the infarct area were collected as free-floating slices in 0.1 M Tris buffered saline (TBS, 0.05 M; pH 7.6).

Supernatants were collected as the cytoplasmic fraction, while the pellet was washed in 200 μL of lysis buffer twice and the pellet was sonicated in 40 μL of Laemlli buffer as the nuclear fraction.

The lumen was first flushed with EDTA PBS buffer, and then, tissue was collected and homogenized in PBS buffer and analyzed as unit per milligram protein (a).

The mitochondria band was collected in MRB buffer and designated as 'mito-pure'.

The floated membrane 'ring' fraction (about 350 µl) was collected, diluted 3 times with buffer A and subjected to ultracentrifugation, as described above.