Sentence examples for buffer was changed for from inspiring English sources

After 4 h at 4°C, the dialysis buffer was changed for 2 L of fresh buffer, and dialysis was left to proceed overnight.

In later analyses the DGVB++ buffer was changed for DGHB++ buffer in which 5 mM HEPES replaced 2.5 mM sodium barbital and the DGVB-Mg-EGTA was changed for DGHB-Mg-EGTA, in which 4.2 mM HEPES replaced 2.1 mM sodium barbital EA cells were prepared as described by Whaley and North [ 25].

The buffer was changed at regular intervals of 8 hrs for 48 hrs.

After the buffer was changed to separation buffer, the mixture was incubated at 37°C for 10 min.

The reaction buffer was changed to a fresh one, and the gels were incubated at 37°C for 24 hours.

Buffer was changed because it is difficult to get veronal buffer in some laboratories.

During concentration of the cleaved protein the buffer was changed to gel filtration buffer.

The dialysis buffer was changed 3 4 times over 24 hr.

The buffer was changed every 2 3 days to maintain pH.

At the third day after transfection, the medium was changed for lysis buffer (50 mM Tris, 2 mM MgCl2, 150 mM NaCl and 0.1% Triton X-100).

Subsequently, the organ bath solution was changed for a fresh pre-heated MOPS buffer and vascular functions were analyzed.