Sentence examples for buffer was changed at from inspiring English sources

The buffer was changed at regular intervals of 8 hrs for 48 hrs.

After preincubation the buffer was changed and the islets were incubated at 1 or 20 mmol/l glucose for 60 min at 37°C unless otherwise stated.

The MPP+-containing buffer was changed once after 2 days and at 5 days post-fertilization (dpf), the larval zebrafish were examined under a fluorescence microscope.

After the buffer was changed to separation buffer, the mixture was incubated at 37°C for 10 min.

After 4 h at 4°C, the dialysis buffer was changed for 2 L of fresh buffer, and dialysis was left to proceed overnight.

The reaction buffer was changed to a fresh one, and the gels were incubated at 37°C for 24 hours.

Finally, the buffer was changed to the measuring buffer 1 × phosphate-buffered saline (PBS) pH 7.4 using ultrafiltration Amicon 3k filters (Milipore).

Dialysis buffer was changed after 2 h and the protein was further dialysed overnight at 4 °C.

The solid gels were put into the lysis buffer (0.01 M Tris, 0.45 M EDTA, pH 7.8, 2% SLS, 10 µg/ml proteinase K) and incubated at 50°C for 40 h, during which the buffer was changed twice.

The buffer was changed daily as to ensure the buffer was not saturated with either SNAP nor silicone oil.

Depolarizing buffer was changed to hyperpolarizing buffer (1 mM KCl, 1 mM CaCl2, and 10 mM MES-Tris at pH 5.6).