Sentence examples for buffer was boiled for from inspiring English sources
Finally, the mixture of beads and SDS loading buffer was boiled for 10 min, and the supernatant was collected.
Before centrifugation at 10,000 g for 5 min at 4°C, the sample buffer was boiled for 5 min.
Twenty-four hours after transfection, cells were lysed in RIPA buffer, containing protease inhibitor cocktail at 4°C for 30 min, spun down at 12,000 rpm for 10 min. Protein extracts in 40 μL of 2× SDS-PAGE sample buffer were boiled for 10 min. Samples were separated on SDS-PAGE gel and transferred to NC membrane.
Subsequently, two volumes of each skeletal muscle homogenate and one volume of SDS-sample buffer were boiled for 4 min. Next, 13% polyacrylamide gels containing 0.1% SDS were loaded with equal amounts of protein from each sample, and electrophoresis was performed using a Mini-Protean 3 Electrophoresis Cell Bio-Rad Laboratoriess, Hercules, CA).
30 μg of cell lysates (1 % Nonidet buffer) were boiled for 10 min at 60 °C in Laemmli sample buffer.
Protein G-precipitated protein complex was recovered by centrifugation and harvested beads resuspended in 30 μl of 2 × SDS PAGE sample buffer were boiled for 5 min.
Protein (50 μg) in 2× Laemmli SDS sample buffer were boiled for 5 min and after centrifugation loaded onto a SDS-PAGE gel.
The lysates with Sodium dodecyl sulphate (SDS) buffer were boiled for 5 minutes and separated by 10% SDS polyacrylamide gel electrophoresis (SDS-PAGE).
Samples of 50 μg protein in loading buffer were boiled for 5 min and loaded on SDS 12 %-polyacrylamide gel electrophoresis.
The Transfection cell lysates/platelet cell lysates in 1X Laemelli buffer were boiled for 5', clarified by centrifugation at 14 000 RPM for 5', loaded onto a 6% SDS PAGE, and transferred onto immobilon nylon membrane.
