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After incubation, 30 μL of excess DAPI-permeabilization buffer was aspirated from the wells.
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Krebs buffer was aspirated, and the cells were incubated with 300 μL of [18F]DCFPyL in 0.9 % NaCl (0.1 MBq ) for 60 min at 37 °C.
Loading buffer was aspirated and replaced with assay buffer.
Excess buffer was aspirated leaving approximately 50 μl of buffer and worms in each tube.
The following day, the blocking buffer was aspirated and washed in PBS-T.
All free buffer was aspirated, and the wet antibody-HLA beads were immediately stored at −80 °C.
Buffer was aspirated and the buffy coat was collected and added in 6.5 mL of total blood.
Antibody in blocking buffer was aspirated and embryos were washed in 4 15 minute PBT washes at RT.
The buffer was aspirated, and each well was washed 3 times with 300 μL wash buffer [phosphate-buffered saline (PBS) with 0.05% Tween-20, pH 7.4].
Then the medium was aspirated from each well and washed 3 × with 250 μl 1 × wash buffer (10 × wash buffer was from CytoSelect tumour-endothelium adhesion assay).
To assess IFN-β induction using a dual luciferase assay, cells were harvested and lysed as follows: Growth media was aspirated from each well, and 200 μl of passive lysis buffer (Promega) was added.
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CEO of Professional Science Editing for Scientists @ prosciediting.com