Sentence examples for buffer was applied for from inspiring English sources

Renatured mda-7/IL-24 protein was achieved by dialysis (the concentration of mda-7/IL-24 was 0.10 mg/mL at the beginning of dialysis) with the decrease of urea concentrations (6, 3, 1.5, 0.75, and 0 M), where each dialysis buffer was applied for 12 h at 4°C with mild agitation.

After cooling, monoclonal mouse anti-human CD68 clone PGM1 (DAKO, Denmark, 1∶25) in 1X wash buffer was applied for 45 minutes (Dako) at room temperature.

A 1 : 100 dilution of α-LmjMCA antibody in TB buffer was applied for 1 h at room temperature.

However, the higher pH (5.5 8.0) of the incubation buffer was applied for phosphoprotein enrichment since the lower pH (that is used for phosphopeptide enrichment) caused protein precipitation (Machida et al. 2007).

After extensive wash the secondary antibodies (in blocking buffer) were applied for 1.5 hours at RT in darkness.

Either rat anti-mouse CD31 or isotypic antibodies 1∶100 in 10× diluted blocking buffer were applied for 1.5 h and then sections were washed with PBS.

Phalloidin-Alexa Fluor 568 (Invitrogen A12380, diluted 1 50 in blocking buffer) and/or BrdU-FITC (Becton-Dickinson 347583, diluted 1 4 in blocking buffer) were applied for 30 min before final washes and mounting in Vector-shield containing 0.5 mg/ml DAPI (Sigma D9542).

One µg/mL each of anti-TfR1 (Invitrogen) or anti-actin (Sigma) antibody, diluted in blocking buffer, was applied to the blot for 1 hour at room temperature.

Sections were rinsed with several changes of PBS+0.02% Triton X-100; anti-rabbit Alexa-Fluor-568-conjugated secondary antibody (diluted 1 400 in blocking buffer) was applied to the sections for 2 hours at room temperature.

After incubation, the plate was washed as above, and alkaline phosphatase-conjugated goat anti-rabbit IgG, diluted 2000-fold in assay buffer, was applied and plates were incubated for 15 min.

One hundred and fifty microliters of binding/washing buffer was applied to the column and incubated for 5 min at 4°C on a rotator.