Sentence examples for buffer was applied as from inspiring English sources

The following buffer was applied as an equilibration buffer in this work: Tris (1 M), pH 8.8 5 mL, 36.03 g urea, 30 mL 100% glycerol, 20 mL 10% SDS, 200 μL 1% Bromophenol blue, and deionized water up to 100 mL.

Subsequently, 5 % normal goat serum blocking buffer was applied.

The buffer was applied using the dropper provided.

The dilution buffer, PBS, was applied as the control to LPS or recombinant human semaphorin 4A (rhSema4A).

Antigen-retrieval microwave treatment of tissues slides in 0.01 mol l−1 citrate buffer pH 6,0 was applied as required.

Penicillin G (Sigma) dissolved in 10 mM sodium phosphate buffer pH 6.8 was applied as a control.

Briefly, antigen-retrieval microwave treatment of tissues slides in 0.01 mol l−1 citrate buffer pH 6.0 was applied as required.

A mixture of sodium carbonate, sodium hydrogen carbonate and sodium sulfate (1∶2∶2, Merck KGaA) was applied as buffer for neutralization after acid catalyzed transesterification.

Phosphate Buffered Saline (PBS; 0.01 M) pH 7.4/0.5 M sodium chloride/0.1% TritonX-100 was applied as a binding buffer and PBS pH 7.4/0.5 M sodium chloride as a wash buffer.

Tris-glycine was applied as electrophoresis buffer, and separation was carried out at 100 120 V.

After washing in Tris-buffered saline tween-20 (TBST) primary antibody was applied as shown in Table 2, and incubated overnight at 4°C.