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TE buffer was analyzed as blank.
For each analysis, the same amount of TE buffer was analyzed as the blank.
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Microsatellite instability (MSI) was analyzed as well.
Luciferase expression was analyzed as above.
As a reference, SDS solutions in the same buffer were analyzed at 0.1 50 mM (cmc at 6 8 mM).
Then the pellet was added 200 μl of Laemmli buffer and sonicated for 10 min. These extracts were analyzed as above described.
Arabidopsis data were analyzed as follows.
After centrifugation at 13 000 r.p.m. for 15 min at 4 °C, the supernatant was analyzed as a soluble fraction and the pellet was resolubilized in 5 M guanidine buffer.
Serum was analyzed as described below.
In addition a series of eight samples of buffer only (PBS) were analyzed as blanks.
Reactions were stopped by the addition of formamide-containing buffer, and products were analyzed as described previously (18).
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