Sentence examples for buffer was added into from inspiring English sources

The 2× SDS-PAGE loading buffer was added into the washed precipitates to elute the samples.

Volumes were adjusted to 100 µL with carbonate-bicarbonate buffer and 150 μL of a reaction buffer was added into each well.

Then, 100 μL of streptavidin-HRP (diluted at 1 5000 in PTM blocking buffer) was added into each well and incubated for 1 h at RT, 600 rpm to detect antigen-antibody binding.

After 24 and 48 h, 20 μL of MTT solution (5 mg/mL in PBS buffer) was added into each well and incubated for another 4 h.

After 48 h, 20 μL of MTT solution (5 mg/mL in PBS buffer) was added into each well and incubated for another 4 h.

As 1 μL of stop buffer was added into the medium to stop the reaction, shRNA from NPs was extracted according to the above method and the products were analyzed by agarose gel electrophoresis.

After 30 min shaking at 4°C, viruses at a multiplicity of infection (MOI) of 500 VP/cell in 50 µl binding buffer were added into each tube, and they were shaken at 4°C for 1.5 hours.

For the internal control of β-gal activities, 90 µl of assay reagent (4 mg/ml ONPG, 0.5 M MgSO4, β-mercaptoethanol and 0.4 M sodium phosphate buffer) were added into each well.

Agarose-conjugated anti-p-Tyr (pY99) antibodies (Santa Cruz Biotechnology Inc., USA, CA) (prewashed three times with IP buffer) were added into each peptide mixture and incubated overnight at 4°C with gentle rotation.

60 μl elution buffer is added into the wells of the filter plate.

Then, 40 60 μl of buffer A and 40 μl of T-cell suspension (∼1×10 2×10 cells/ml) in the same buffer were added into the small chamber.