Sentence examples for buffer was added in from inspiring English sources

The reaction was allowed to react for 15 min before 15 mg (0.02 mmol) of 9, dissolved in 2 mL of 0.05 M phosphate buffer, was added in one portion to the reaction mixture.

Subsequently 0.5 µg of biotin labeled DBS diluted in 50 µl 1X GMS buffer was added in the well and incubated at 4°C for 1 hour.

The buffer was added in 1 ml per 1 g of cells ratio to 1.5 ml Eppendorf tubes, which we vigorously shook five times for one minute in a Disruptor Genie (Scientific Industries Inc ,Bohemia, NY, U.S.A).

Extraction buffer was added in each extract before heating.

Briefly, 600 µl of borate extraction buffer was added in the powder and left overnight in a shaker.

Serum diluted 1 200 in RIA buffer, was added in duplicate, and incubated for 1.5 h at room temperature.

In order to reduce the signal reflection, output buffer is added in this design.

Subsequently, an optimal concentration of fluorochrome-conjugated antibodies (between 0.06 0.5 µg/1×106 cells in 50 µl of FACS buffer) were added in various combinations to allow for dual or triple staining experiments and plates were incubated for 30 minutes on ice.

According to manufacturer's instructions, 1U of DNase and 1 µl of DNase 10× Reaction Buffer were added in 8 µl of RNA sample and incubated 30 minutes at 37°C.

Serum samples, diluted 1 50 in incubation buffer, were added in duplicate (100 μl/well) and incubated for two hours at room temperature.

50 μL of dye-labeled CPMV and TMV diluted in 0.1 M potassium phosphate buffer were added in triplicate to a black 384-well plate at concentrations of 2.5 μM sCy5, 50 nM CPMV, or 5 nM TMV.