Sentence examples for buffer was added for from inspiring English sources

No buffer was added for pH stabilization.

The vial was heated and optionally buffer was added for pH-adjustment.

Seventy microliters of lysis buffer was added for every 20 µl of packed cell volume.

The homogenate was transferred into a precooled 40 ml tube, and 6 ml of extraction buffer was added for two times to clean the mortar.

Following three washes with TBS/T, secondary antibody (Table 2) diluted 1∶4000 in blocking buffer was added for 1 hr at room temperature and washed three times with TBS/T.

Following an incubation of the reaction mixture at 30°C for 10 min, 2% SDS-containing or urea-containing sample buffer was added for either the 1D or 2D gel electrophoresis analysis, respectively.

Alexa Fluor-conjugated secondary antibodies, diluted 1 200 in blocking buffer, were added for 1 hour at room temperature.

After an incubation time of 10 min at room temperature, additional 400 μl of binding buffer were added for a final volume of 500 μl.

After three washes, anti-mouse or rabbit Europium-labeled secondary antibodies (1 μg/ml in assay buffer) were added for an additional hour.

Wells were washed twice with SM buffer and either 220 μl of a phage stock (10 PFU/ml) or 220 μl of SM buffer were added for test and control purposes, respectively.

The frozen whole blood with anticoagulant was thawed in a 37°C water bath and 4 volumes of red blood cell lysis buffer were added for lysing red blood cells.