Sentence examples for buffer was added as from inspiring English sources
10 μL of 5x reaction buffer was added, as well as 2.5 μL of denaturation solution, then the mixure was gently mixed.
After incubation for 48 h in the dark at 37°C, MTT lysis buffer was added as described previously.
To the fourth well containing plasma, 175 μL phosphate buffer was added as a blank.
The plasma was removed, and a dilution buffer was added as described.
The pulverized tissue was not allowed to thaw before the buffer was added, as this could have triggered RNA degradation.
Then 100 μl 0.05 M iodoacetamide in UA buffer was added as a blocking agent and the samples were incubated for 20 min in darkness.
In this assay a solution containing pepsine treated polyclonal IgG Freeze buffer is added, as a result anti-allotype antibodies are not detected.
Diluted and neutralized transgenic mouse brain homogenates, neat resuspended transgenic plasma samples or transgenic mouse CSF samples (diluted 1 10 in MSD blocking buffer) were added as described above to blocked MSD 96-well multi-spot Aβ triplex ultra-sensitive ELISA plates with SULFO-TAG 6E10 antibody (MSD).
Approximately 2.5 µg of purified recombinant protein was supplemented with 1% SDS and incubated at 95°C for 5 min. After cooling on ice, 1.0 µL PNGase F (NEB, USA) or water was added as well as buffer (final concentration of 50 mM sodium phosphate, pH 7.5) and 1% Nonidet P-40 detergent in a total volume of 20 µL.
Sigma RBC lysis buffer were added at room temperature.
Sec17p (600 nM), Sec18p (200 nM), and HOPS (100 nM) or their respective buffers were added as indicated, and the reactions were assayed for protected lumenal compartment mixing.
