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ChonBlock™ blocking buffer was procured from Chondrex, Inc. (Redmond, WA).
Buffer was subsequently chilled at 4 °C.
The elution buffer was D-PBS.
Then, ATP assay buffer was added.
100 µL of extraction buffer was used as a control.
The buffer was then replaced with ATP assay buffer.
Sample buffer was added to 1× final concentration.
20 µL TAE/Mg2+ buffer was further added.
A second 300 sec baseline in kinetic buffer was performed.
The buffer was changed daily as to ensure the buffer was not saturated with either SNAP nor silicone oil.
A two-goal buffer was then established after Catriona Wagg converted another chance.
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