Figure 13 Effect of buffer volume on fluorescence quenching of eosin by EBS (EBS = 1 μg/ml).
The extraction time was not significant, but the linear, and the quadratic effects of the buffer volume were significant on the enzyme activity.
The entire 96FASP procedure prior to overnight digestion, including urine concentrate denaturation, alkylation and intermittent centrifugation steps, requires 4 6 h depending on the UA buffer volume, the total protein amount loaded and possibly the presence of other macromolecular substances.
The effect of mcliavain buffer volume (pH 2.2) on the fluorescence quenching was also investigated.
There are other uneven plots, which reflect the effect of variables like buffer pH, buffer volume and temperature on the activity of lipase employed.
Whole specimens were placed in 2 ml Eppendorf Biopur tubes, fully immersed in digestion buffer (volume dependent on specimen size, in our experiments 0.5 0.7 ml), and incubated overnight at 55°C with gentle agitation.
DNA was extracted from museum specimens and macrofossils using the non-destructive method [16]: Whole specimens were placed in 2 ml Eppendorf Biopur tubes, fully immersed in digestion buffer (volume dependent on specimen size, 0.5 1.5 ml in this study), and incubated overnight at 55°C with gentle agitation.
Then proteins, ATP and/or GTP (depending on experiment) of defined concentration were added to a buffer volume of 200 µl on top of the supported lipid membranes.
The factors included pH, type of buffer, volume of buffer, concentration of the reagent, reaction time and volume of acetonitrile.
The role of the buffer volume as an acoustic filter is investigated and optimized dimensions of the buffer volume, to achieve minimum noise transmission coefficient, are calculated.
