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After processing, the tubes were centrifuged at 16,000×g for 5 min and an aliquot depending on the amount of soil and buffer volume of the supernatant fluid was transferred into a fresh sterile 2-ml microtube.
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The effect of buffer volume on the mercury removal of the petrochemical waste (0.1872 µg mL−1) by the present magnetic adsorbent (MIONs) was investigated.
The factors included pH, type of buffer, volume of buffer, concentration of the reagent, reaction time and volume of acetonitrile.
Then proteins, ATP and/or GTP (depending on experiment) of defined concentration were added to a buffer volume of 200 µl on top of the supported lipid membranes.
The higher the hypotonic buffer volume, the lower the resulting concentration of the hemoglobin was in the resealed cells.
After three washing steps using RIPA buffer, the volume of the Sepharose beads was adjusted with H2O to 100 µl and RNA was extracted using the RNeasy kit (Qiagen).
For 2 volumes of worms washed with M9 buffer, 1 volume of the bleach solution was added.
Besides, the flexible and highly-conductive coating layer buffers volume change of the cathode during discharge-charge process and reduces charge transfer resistance.
Numerous open pores, which could buffer volume expansion of silicon and improve the electrode kinetics, are engendered in the microspheres due to the rapid evaporation of solvent and unfoldment of flexible MWCNTs.
The extraction time was not significant, but the linear, and the quadratic effects of the buffer volume were significant on the enzyme activity.
Samples of sarkosyl-insoluble tau fractions [ 23] were dissolved in 1× SDS sample loading buffer in 1/50 volume of the soluble fraction and heated at 95°C for 5 minutes.
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