Sentence examples for buffer transfer from inspiring English sources

After adding 100  μL of Pinpoint Binding Buffer, transfer to a Zymo-Spin1 Column and span the Column at 12000 rpm for 10 seconds.

Plasma or supernatant from cultured tissue synoviocytes of the CIA rats was subjected to Western blotting using NuPage 4 12% Bis-Tris gels, MOPS running buffer, transfer buffer, and 0.2 μm PVDF membrane (Invitrogen), in accordance with the manufacturer's protocol.

The preparation of the conjugates themselves can be time-consuming since it requires a buffer transfer step to remove reducing agents (which are usually present in the storage buffer of a protein which contains free cysteines), following which the protein must be incubated with the conjugate for 1 or more hours, and then a 2nd buffer exchange must be carried out to remove unreacted conjugate.

Beads were washed twice with 250µl of NT-2 buffer, transferred to a micro-spin column (Pierce) and washed four more times with 200µL of NT-2 buffer.

The resulting pellets were then re-suspended in 1.5 ml wash buffer, transferred to 2 ml microcentrifuge tubes and centrifuged (100×g, 5 min).

Magnetically separated immune complexes were eluated with 1× SDS gel loading buffer, transferred to nitrocellulose, and blocked overnight in washing buffer with 3% BSA and 2 µg/ml streptavidine.

After washing twice with the wash buffer, cells were resuspended in 100 µl wash buffer, transferred in a microtiter plate and fluorescence intensity was measured at excitation 485 nm and emission 530 nm.

The globes were dissected free of periocular connective tissue, rinsed with HBSS buffer, transferred into 2% dispase (Roche, Nutley, NJ) in HBSS, and placed in an incubator at 37°C for 45 60 minutes.

The supernatants were removed and kept on ice in separate tubes, while the pellets were suspended in 350 µl RLT buffer, transferred to new FastPrep tubes and subjected to another round of homogenization.

Gravid adults from these boxes were then harvested by several rinses with M9 buffer, transferred to 25 ml conical tubes and pelleted by centrifugation for 3 min at 3000 rpm.

The tubes were centrifuged for 3 minutes at 4000 g, the extraction buffer transferred into a Centricon 30 (Millipore Corp., Bedford, MA) and then concentrated until the volume had decreased to 100 ul.