Sentence examples for buffer to the final from inspiring English sources

Within a buffer family, memory is allocated to buffers in a logarithmically decreasing manner from the receiving buffer to the final aggregate buffer.

On the day of experiments the stock solution was thawed and diluted in Ringer buffer to the final concentrations.

NPLC0393 was dissolved in DMSO as a stock solution and diluted in reaction buffer to the final concentration.

Non-displaceable binding was assessed by adding dextran-coated charcoal (Sigma) admixed with 1% ovalbumin in HEDG buffer to the final concentration 0.15%.

Then, plasmidic DNA was serially diluted in a stabilizing buffer to the final desired concentration.

The cell suspension were mixed with same volume of the labeling solution containing PKH26 in a dilution buffer to the final concentration of 4 μ m.

Samples spanning 0.1 1.5 OD at A230, A250, or A280 (Supporting Information, SI-Table 3) were prepared in a total volume of 500 μL by mixing concentrated protein solution with reference buffer to the desired final concentration for each experiment.

Pellets were resuspended in different amounts of the same buffer to adjust the final OD600 to ∼0.2.

Briefly, undiluted anti-Pis1 antibody (727 μg⋅ml-1, according to GenScript) was mixed with an equal volume of each peptide solution, maintaining a ratio of 1 2 for the concentration of the antibody and the peptide, and incubated at 37°C for 45 min. The pre-absorbed antibody was later diluted in dilution buffer to make the final concentration of ~14.5 μg⋅ml−1 before being used for IHC.

The pellet was subjected to a further five washes with wash buffer to give the final inclusion body sample.

Each translation reaction contained 50% in vitro translation lysate (from 293T cells) and buffer to make the final reaction 0.84 mM ATP, 0.21 mM GTP, 21 mM creatine phosphate (Roche), 45 U/ml creatine phosphokinase (Roche), 10 mM HEPES-KOH, pH 7.6, 2 mM DTT, 8 mM amino acids (Promega), 255 mM spermidine, 1 U/ml murine RNase inhibitor (NEB), and mRNA-specific concentrations of Mg OAc 2 and KOAc.