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pH was raised to 6 with phosphate buffer to stop the reaction by OH scavenging at elevated pH, and it also made Fe2+ unavailability by converting into Fe3+.
Finally, the sections were incubated with 0.1 M TRIS buffer to stop the reaction.
Samples were taken at different time points and directly mixed with 4x SDS sample buffer to stop the reaction.
Sections were then rinsed in buffer to stop the reaction, followed by PBS washing and examined under light microscope (Nikon Eclipse 80i, Shinagawa-Ku, Tokyo, Japan).
An aliquot of the reaction was taken out at different time points and mixed with SDS sampling buffer to stop the reaction.
At intervals, an aliquot was cooled quickly by 20-fold dilution in cold sodium phosphate buffer to stop the denaturation reaction and the remaining activity was recorded.
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They want Mexico to be the buffer, to stop arrivals before they get to our border".
Afterwards, the chromosomes were rinsed in SSC buffer again to stop the denaturation process and to stabilize their structure.
25 µl of reaction mix were taken at different time intervals and mixed with an equal volume of Laemmli reducing sample treatment buffer immediately to stop the reaction.
400 μL of 1X binding buffer was added to stop the staining reaction.
An equal volume of 2× SDS sample buffer was added to stop the reaction and the samples were boiled for 10 minutes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com